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It is possible to use a variety of techniques to protect against clinical disease in rabbits. Passive immunisation has several advantages and it is possible to protect rabbits with human monoclonal antibodies. We have used the monoclonal antibody GQ1b to protect rabbits against oral challenge with lethal strains of S. enterica serovar Typhimurium. This antibody is a murine monoclonal antibody that reacts with the lipopolysaccharide G epitope of S. enterica serovar Typhi, recognised by human and rabbit antibodies. The antibody was produced by the injection of mice with killed S. enterica serovar Typhimurium in Freund's adjuvant, followed by a fusion of splenocytes with mouse myeloma cells. The antibody was purified by immunoadsorption and ion exchange chromatography. Rabbits were immunised with GQ1b antibody at 1 mg/kg body weight, in Freund's adjuvant, subcutaneously, at intervals of two weeks. The rabbits were challenged orally with 01e38acffe
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